Travel travel travel!

I'm off on a road trip tonight, so the godawful headache that's been plaguing me for the past few days had better be on its way out!

In other travel-related business, I'll be heading to Europe with my family for Golden Week, starting in Vienna and ending in Berlin. We'll be renting a car, and plan on hitting up Prague, Ceske Krumlov and Regensburg, with various side excursions. B1 is getting antsy and wants to start planning our itinerary, so I turn to you guys: is there anything in any of those towns, or other towns nearby, that we mustn't miss? I've been to Vienna, Prague and Ceske Krumlov before, but I'd love your input, especially if it's about what/where to eat. ^o^

Thanks!